When it comes to sampling a specific solo tree in your environment, ensuring that you are targeting the right area is crucial for obtaining accurate results. One key aspect to consider is sampling within the "Drip Line" or "Drip Halo" surrounding the base of the tree. This area, often referred to as the zone where rainwater drips off the outermost leaves and branches, is where a significant amount of nutrients, organic matter, and root exudates accumulate. By sampling within this zone, you are more likely to capture a representative snapshot of the tree's nutrient uptake and overall health.

Sampling outside the Drip Line may not provide an accurate reflection of the tree's nutrient status, as the concentration of essential elements and other compounds can vary significantly within the root zone. Moreover, sampling too close to the trunk may not capture the full extent of the tree's root system and nutrient uptake capacity. Therefore, by focusing on the Drip Line area, you are targeting a region that is biologically active and directly influenced by the tree's physiology.

In addition to the Drip Line, considering factors such as soil type, moisture levels, and proximity to other trees or structures can further refine your sampling strategy. By taking a comprehensive approach to sampling around a solo tree, you can gather valuable insights into its nutritional needs, growth patterns, and overall well-being, ultimately enabling you to make informed decisions regarding its care and maintenance.

Soil Sampling Protocol for the Biological Approach to Growing Plants.

Objective

To collect soil samples that accurately represent the biological diversity and health of the soil in a given area, using a randomized core collection method and mapping of collection points.

Equipment Needed

- MBG Soil Core Puller (Kitbox included).

- Collection Kitbox (included mailer).

- MBG Pen

- GPS device or smartphone with GPS capability

- Camera or smartphone with camera

- Gloves (optional)

- Notebook and pen

Preparation

1. Create a Collection Map: Draw a map of the sampling area, marking potential sampling points. Ensure these points are randomly distributed across the area to avoid bias.

2. Identify Sampling Area: Define the boundaries of the area to be sampled. Notate them on your drawing/map/photo. Identify the target of this test. This could be a field, garden, solitary tree, or any other designated plot.

Sampling Procedure

Step 1: Randomized Sampling Points

1. Randomize Collection Points: Use a random selection of the sample collection points in your project area.

2. Mark Points on your Map: Plot these points on your collection map.

3. Take Photos: Make sure you take photos to correspond with the sampling points on your map. Also photograph the soil surface at each of these locations to upload to your sample file. These photos will help us to help you. The more information we have to work with, the better we can help you.

Step 2: Core Collection

1. Prepare Equipment: Ensure the MBG Soil Core Puller is clean and ready for use.

2. Complete Sample Registration Document: Make a note of the Registration Number on the document with the collection box to register your sample with us online.

Step 3: Collecting the Samples

1. Navigate to Sampling Point: Use the GPS device to locate the first randomized sampling point.

2. Document Location:

   - Take a close-up photo of the exact spot where you will insert the soil core puller.

   - Take a full photo showing the broader context of the sampling location.

3. Insert Soil Core Puller:

   - Insert the soil core puller into the soil.

   - Rotate and pull out the core, ensuring a consistent sample depth.

4. Transfer to Soil Sample Kitbox: Place the soil core into the corresponding labeled collection box.

5. Record Details: In your notebook, record the GPS coordinates, date, time, and any notable observations about the sampling location.

Step 4: Repeat Process

1. Move to Next Point: Repeat the above steps for each randomized sampling point.

2. Document Each Point: Ensure each sampling point is documented with photos and recorded details.

Post-Sampling

Step 1: Review Collection Map

1. Verify Completeness: Ensure all randomized points have been sampled and documented on your collection map.

Step 2: Store Samples

1. Seal Boxes: Ensure all collection bags are properly sealed to prevent contamination.

2. Store Samples: Store the samples in a cool, dark place until they can be shipped.

Step 3: Data Management

1. Organize Photos: Label and organize the photos corresponding to each sampling point.

2. Compile Data: Compile all recorded data, photos, and the collection map into a single report for analysis.

Analysis

- Send Samples for Analysis: Send the soil samples to our lab specializing in soil food web analysis to assess biological diversity and soil health.

- Review Results: Use the results to inform soil management and plant growth strategies based on the biological approach.

This protocol ensures a thorough and unbiased collection of soil samples, providing valuable insights into the biological health of your soil. Proper documentation and randomization are key to obtaining accurate and representative data.