Soil Sampling Protocol for the Biological Approach to Growing Plants.

Objective

To collect soil samples that accurately represent the biological diversity and health of the soil in a given area, using a randomized core collection method and mapping of collection points.

Equipment Needed

- MBG Soil Core Puller (Kitbox included).

- Collection Kitbox (included mailer).

- MBG Pen

- GPS device or smartphone with GPS capability

- Camera or smartphone with camera

- Gloves (optional)

- Notebook and pen

Preparation

1. Create a Collection Map: Draw a map of the sampling area, marking potential sampling points. Ensure these points are randomly distributed across the area to avoid bias.

2. Identify Sampling Area: Define the boundaries of the area to be sampled. Notate them on your drawing/map/photo. Identify the target of this test. This could be a field, garden, solitary tree, or any other designated plot.

Sampling Procedure

Step 1: Randomized Sampling Points

1. Randomize Collection Points: Use a random selection of the sample collection points in your project area.

2. Mark Points on your Map: Plot these points on your collection map.

3. Take Photos: Make sure you take photos to correspond with the sampling points on your map. Also photograph the soil surface at each of these locations to upload to your sample file. These photos will help us to help you. The more information we have to work with, the better we can help you.

Step 2: Core Collection

1. Prepare Equipment: Ensure the MBG Soil Core Puller is clean and ready for use.

2. Complete Sample Registration Document: Make a note of the Registration Number on the document with the collection box to register your sample with us online.

Step 3: Collecting the Samples

1. Navigate to Sampling Point: Use the GPS device to locate the first randomized sampling point.

2. Document Location:

   - Take a close-up photo of the exact spot where you will insert the soil core puller.

   - Take a full photo showing the broader context of the sampling location.

3. Insert Soil Core Puller:

   - Insert the soil core puller into the soil.

   - Rotate and pull out the core, ensuring a consistent sample depth.

4. Transfer to Soil Sample Kitbox: Place the soil core into the corresponding labeled collection box.

5. Record Details: In your notebook, record the GPS coordinates, date, time, and any notable observations about the sampling location.

Step 4: Repeat Process

1. Move to Next Point: Repeat the above steps for each randomized sampling point.

2. Document Each Point: Ensure each sampling point is documented with photos and recorded details.

Post-Sampling

Step 1: Review Collection Map

1. Verify Completeness: Ensure all randomized points have been sampled and documented on your collection map.

Step 2: Store Samples

1. Seal Boxes: Ensure all collection bags are properly sealed to prevent contamination.

2. Store Samples: Store the samples in a cool, dark place until they can be shipped.

Step 3: Data Management

1. Organize Photos: Label and organize the photos corresponding to each sampling point.

2. Compile Data: Compile all recorded data, photos, and the collection map into a single report for analysis.

Analysis

- Send Samples for Analysis: Send the soil samples to our lab specializing in soil food web analysis to assess biological diversity and soil health.

- Review Results: Use the results to inform soil management and plant growth strategies based on the biological approach.

This protocol ensures a thorough and unbiased collection of soil samples, providing valuable insights into the biological health of your soil. Proper documentation and randomization are key to obtaining accurate and representative data.

We used to use plastic bags when we first started taking samples, so instead use the MBG Sample Kitbox to put your pulled sample cores into.

When pulling samples from a single container we have to take note of a few things. Make sure there is enough soil in this container for you to be able to pull enough material for a sample. For the smallest, tiniest sample conditions we would need at least enough of the soil medium to work with. That would approximately equate to about one full soil core. So use the included MBG Soil Core puller to measure you are giving us at least that much.

Another major factor when trying to sample our most beloved tiny plants is getting the sample material while creating as little damage to the rootball as possible. We often suggest that you time or schedule pulling a sample for a small container at the same time you have scheduled repotting the specimen. That way, any damage can be mitigated by the additional hopefully healthy soil you are adding into the bigger container.

When pulling samples from a single container, it is crucial to pay attention to specific details to ensure the accuracy and reliability of the sample. First, it is essential to confirm that the container has an adequate amount of soil to extract sufficient material for the sample. For the most precise sampling conditions, it is recommended to have enough soil medium that would be equivalent to at least one full soil core. Use the MBG Soil Core puller to measure the amount of soil being extracted to confirm it meets the required quantity.

Another critical consideration when sampling delicate small plants is to minimize any potential damage to the rootball during the sampling process. One effective strategy is to coordinate the sampling of a small container with the scheduled repotting of the plant. By aligning these activities, any damage incurred during sampling can be offset by introducing fresh, healthy soil into the larger container during the repotting process. This approach helps in preserving the overall health and integrity of the plant while obtaining the necessary sample for analysis. Be careful not to damage roots and to remove any uncomposted organic matter from the sample. Make sure your sample consists of soil medium and not mulch and other organic surface particles.