When it comes to sampling up to 3/4 of an acre, the protocols remain consistent with those for 1/4 acre sampling. It is essential to ensure that you refer to the information provided for the 1/4 acre sampling protocols to gain a comprehensive understanding. As you extract sample cores, it is crucial to maintain a high level of organization throughout the process. Our goal is to utilize the samples and data you supply to offer you valuable insights into the biology of your land.

For each Sample Box that you send to us, it should contain a representative aggregate sample of the same type of soil. For instance, if you are sampling 3/4 of an acre of grasslands, you can compile a sample box that includes cores from the entire area. However, it is important to segment your acreage into biologically sampled sections accordingly. For example, if you have 3/4 of an acre dedicated to orchard and 1/4 acre to grassland, you can divide this into two MBG Lab tests, resulting in two MBG Sample Kitboxes.

One box should encompass the 3/4 acre of orchard sample cores, while the other box should contain the 1/4 acre sample cores from the grassland separately. By ensuring that your testing is based on "representative" samples, you have the flexibility to test more than 1/4 acre using a single MBG Sample Kitbox. This approach allows for a more comprehensive analysis of your land's biology, providing you with a wealth of knowledge to make informed decisions and optimize your agricultural practices.

Soil Sampling Protocol for the Biological Approach to Growing Plants.

Objective

To collect soil samples that accurately represent the biological diversity and health of the soil in a given area, using a randomized core collection method and mapping of collection points.

Equipment Needed

- MBG Soil Core Puller (Kitbox included).

- Collection Kitbox (included mailer).

- MBG Pen

- GPS device or smartphone with GPS capability

- Camera or smartphone with camera

- Gloves (optional)

- Notebook and pen

Preparation

1. Create a Collection Map: Draw a map of the sampling area, marking potential sampling points. Ensure these points are randomly distributed across the area to avoid bias.

2. Identify Sampling Area: Define the boundaries of the area to be sampled. Notate them on your drawing/map/photo. Identify the target of this test. This could be a field, garden, solitary tree, or any other designated plot.

Sampling Procedure

Step 1: Randomized Sampling Points

1. Randomize Collection Points: Use a random selection of the sample collection points in your project area.

2. Mark Points on your Map: Plot these points on your collection map.

3. Take Photos: Make sure you take photos to correspond with the sampling points on your map. Also photograph the soil surface at each of these locations to upload to your sample file. These photos will help us to help you. The more information we have to work with, the better we can help you.

Step 2: Core Collection

1. Prepare Equipment: Ensure the MBG Soil Core Puller is clean and ready for use.

2. Complete Sample Registration Document: Make a note of the Registration Number on the document with the collection box to register your sample with us online.

Step 3: Collecting the Samples

1. Navigate to Sampling Point: Use the GPS device to locate the first randomized sampling point.

2. Document Location:

   - Take a close-up photo of the exact spot where you will insert the soil core puller.

   - Take a full photo showing the broader context of the sampling location.

3. Insert Soil Core Puller:

   - Insert the soil core puller into the soil.

   - Rotate and pull out the core, ensuring a consistent sample depth.

4. Transfer to Soil Sample Kitbox: Place the soil core into the corresponding labeled collection box.

5. Record Details: In your notebook, record the GPS coordinates, date, time, and any notable observations about the sampling location.

Step 4: Repeat Process

1. Move to Next Point: Repeat the above steps for each randomized sampling point.

2. Document Each Point: Ensure each sampling point is documented with photos and recorded details.

Post-Sampling

Step 1: Review Collection Map

1. Verify Completeness: Ensure all randomized points have been sampled and documented on your collection map.

Step 2: Store Samples

1. Seal Boxes: Ensure all collection bags are properly sealed to prevent contamination.

2. Store Samples: Store the samples in a cool, dark place until they can be shipped.

Step 3: Data Management

1. Organize Photos: Label and organize the photos corresponding to each sampling point.

2. Compile Data: Compile all recorded data, photos, and the collection map into a single report for analysis.

Analysis

- Send Samples for Analysis: Send the soil samples to our lab specializing in soil food web analysis to assess biological diversity and soil health.

- Review Results: Use the results to inform soil management and plant growth strategies based on the biological approach.

This protocol ensures a thorough and unbiased collection of soil samples, providing valuable insights into the biological health of your soil. Proper documentation and randomization are key to obtaining accurate and representative data.